Ramirez, Ana S.
Ana S. Ramirez
Assistant Professor
Biochemistry & Molecular Biology
Structural and mechanistic investigation of the GPI-anchor biosynthesis pathway
Structural and mechanistic investigation of the GPI-anchor biosynthesis pathway
Our research aims to elucidate the mechanism of membrane transporters, flippases, and polymerases involved in bacterial cell wall biosynthesis, unravel how their function influences bacterial adaptation, discover inhibitory molecules for the development of new antimicrobials, and repurpose the activity of these proteins for applications in the synthesis of glycoconjugates. Our lab uses a multidisciplinary approach, combing techniques such as single-particle cryo-electron microscopy (cryo-EM), x-ray crystallography, live cell assays, and various buichemical/biophysical methods to study the function and molecular mechanism of membrane proteins.
Post-transcriptional regulation of gene expression and control of mRNA-protein dynamics by RNA helicases in normal conditions and cell stress.
Biosynthesis and regulation of plant cell wall synthesis and deposition, post-translational modifications, glycan imaging and dynamics.
Engineering tRNA technology to build novel proteins with selenocysteine.
Molecular and cellular mechanisms of brain tumors; oncohistone mutations; human ES cell-based tumor modeling; tumor heterogeneity and evolution
Structural analysis and hands-on training for all glycoconjugates using mass spectrometry and NMR
Transcriptional and epigenetic mechanisms regulating preimplantation development, pluripotency, differentiation and cellular reprogramming in humans
Our goal is to define the impact of protein post-translational modifications on structure and function in the immune system.
Structure and function of enzymes involved in plant polysaccharide biosynthesis and modification with the long term goal to develop pathway engineering or targeted genomics approaches for enhanced production of bio-materials, -products, and -fuels.